Reference Values for Immature Granulocytes in Healthy Blood Donors Generated on the Sysmex XE-2100 Automated Hematology Analyer

An increase in immature granulocytes (IG) in peripheral blood is of great importance for the diagnosis of hematological malignancies. Furthermore, it may indicate systemic inflammation. IGs are usually quantified by visual microscopy; however, recent studies have evaluated automated IG count on the DIFF channel and IG detection on the IMI channel of the Sysmex XE-2100 automated hematology analyser. The aim of the present study was to generate reference values for these parameters. Men and women showed comparable reference intervals for the IG counts; the 5th percentile was zero and the 95th percentile was 0.03×10/ L for both men and women. For the IG count in percent (%IG) the 5th percentile was zero and the 95th percentile was 0.5% for men and 0.4% for women. The count of myelocytes in the IMI channel in the study population resulted in a 5th percentile of zero and a 95th percentile of 0.03×10/ L for both men and women. The clinical value of these automated measurements has to be elucidated in further clinical studies.